Detection and mapping of Thymallus thymallus in Gesäuse National Park

The primary goal of the thesis was to test the eDNA qPCR protocol. For this are the Enns and Johnsbach in the area of the Gesäuse National Park for sampling have been selected. It has been tested whether graylings (Thymallus thymallus) rise into the Johnsbach or not. The eDNA sampling has five times at 3 times between the end of April and the end of May 2019. Positions of the Enns and in 2 places of the Johnsbach took place. It’s at every rehearsal site a total of 1 litre of water has been pressed through filter to collect the eDNA in it. The Samples were then extracted in the laboratory and tested on the basis of an qPCR, whether therein Ashes of DNA. The protocol has worked in both the Enns and Johnsbach, since at any point at least one replica amplified. It has therefore been found that the Protocol also in larger rivers such as the Enns, in relation to the size of the river the ashes Population density is low, works. Also in Johnsbach, where no grayling awaits few replicas have been amplified, and it can be assumed that ashes at least ascend to the spot of pale stone.