Data set for "Cell-type-specific nicotinic input disinhibits mouse barrel cortex during active sensing"

Data set for: Gasselin C, Hohl B, Vernet A, Crochet C, Petersen CCH (2021) Cell-type-specific nicotinic input disinhibits mouse barrel cortex during active sensing. Neuron doi: 10.1016/j.neuron.2020.12.018 There are 2 files in this upload: 1. The file named "2021_Gasselin_Neuron.pdf" is the Open Access pdf of the online publication in Neuron. 2. The file named "Gasselin_data_code.zip" (~9 GB) is a zipped version of a folder "Gasselin_data_code" (~13 GB), which contains the data analysed in the study along with the Matlab code used to generate the published figures. To access the data and the code, first unzip the file. Then add the folder with subfolders to the Matlab path and run the different codes. The current folder must be the main folder (‘Gasselin_data_code’). Each code computes and plots the results used in the corresponding figure. Figures and Tables are saved in the subfolder ‘Figures’. The subfolder ‘Functions’ contains functions called by the main codes. The main folder contains the following codes: Gasselin_Figure1: computes and plots the results for the panels D, E and F of figure 1. Gasselin_Figure2: computes and plots the results for the panels B and C of figure 2. Gasselin_Figure3: computes and plots the results for the panels B, C and D of figure 3. Gasselin_Figure4: computes and plots the results for the panels A, B and C of figure 4. Gasselin_FigureS1: computes and plots the results for the panels A, B and C of figure S1. Gasselin_FigureS2: computes and plots the results for the panels A and B of figure S2.   The subfolder ‘Data’ contains the data structures used for the different figures: data_figure1.mat data_figure2.mat data_figure3.mat data_figure4_MECA.mat data_figure4_Activation.mat data_figure4_Inactivation.mat data_figureS2_Activation.mat data_figureS2_Inactivation.mat data_Axon.mat   The data structures contain the following fields: Mouse_Name : name of the mouse. Mouse_DateOfBirth: date of birth of the mouse (YMD). Mouse_Sex: sex of the mouse (F or M). Mouse_Genotype: genotype of the mouse. Mouse_Drug: experimental condition of the recording (control = ‘No Drug’; blockade of glutamatergic transmission = ‘CNQX_DAPV’; blockade of glutamatergic transmission and nicotinic receptors = ‘CNQX_DAPV_MECA’; blockade of nicotinic receptors only = ‘MECA’). Mouse_Virus: virus injected if any. Cell_Counter; cell recorded in a given mouse. Cell_Type: type of the recorded cell based on 2P imaging. (EXC, VIP, PV, SST, 5HT3aR_non_VIP). Cell_Depth: depth of the recorded cell relative to pia (µm). Cell_TargetedBrainArea: cortical area targeted (C2 column of the barrel cortex = C2). Cell_Fluorescence: expression of the genetically encoded fluorophore (FALSE or TRUE). A neuron recorded in a VIP_IRES_Cre x LSL_tdTomato (cf Mouse_Genotype) with Cell_Fluorescence=TRUE is considered as a VIP neuron (cf Cell_Type). Sweep_Counter: number of the sweep recorded for a given neuron (data were acquired across successive continuous sweeps of 30-60 s). Sweep_Type: experimental condition during that sweep (Only spontaneous whisking onset = ‘Onset’; Whisking onset and whisker stimulus = ‘Onset_Whisker_Stim’ ; Optogenetic stimulation = ‘Opto_Stim’;  Optogenetic activation = ‘Opto_Activation’; Optogenetic inactivation = ‘Opto_Inactivation’;  ). Sweep_Start_Time: time at the beginning of the sweep recording (YMDHms). Sweep_WhiskerAngle: C2 whisker angular position extracted from simultaneous high-speed video filming (deg). Sweep_WhiskerAngle_SamplingRate: sampling rate of the whisker angle trace. Sweep_WhiskingOnset_Time: time of identified whisking onset - excluding any whisker stimulus shortly before or after (s). Sweep_MembranePotential: membrane potential recording (mV) after cutting of the APs. Sweep_MembranePotential_SamplingRate: sampling rate of the membrane potential signal (pt.s-1). Sweep_CurrentInjected: current injected into the cell (pA). Sweep_CurrentInjected_SamplingRate: sampling rate of current signal (pt.s-1). Sweep_WhiskerStim_Name: whisker to which the magnetic stimulus was applied to (C2 or B2&C2). Sweep_WhiskerStim_Time: onset times of the whisker stimulus (s). Sweep_OptoStim_Power: light power applied for optogenetic manipulations (% of the max power). Sweep_OptoStim_Time: onset times of the light pulses for optogenetic manipulations (s). Cell_ID: unique cell identifier (= Mouse_Name+Cell_Counter). SpikeThreshold: spike threshold used to detect APs (mV). Trial_WhiskingOnset: data structure containing the cut signals used to compute averaged responses around whisking onset times. Trial_WhiskerStim: data structure containing the cut signals used to compute averaged responses around whisker stimulus onset times. Trial_WhiskerStim_QuietTrials: data structure containing the cut signals used to compute averaged responses around whisker stimulus onset times for trials without whisker movements. Trial_WhiskerStim_WhiskingTrials: data structure containing the cut signals used to compute averaged responses around whisker stimulus onset times for trials with whisker movements. Trial_Opto: data structure containing the cut signals used to compute averaged responses around optogenetic stimulus onset times. Trial_OptoAndWhisker_QuietTrials: data structure containing the cut signals used to compute averaged responses around whisker stimulus onset times for trials with optogenetic manipulation and no whisker movements. Trial_OptoAndWhisker_WhiskingTrials: data structure containing the cut signals used to compute averaged responses around whisker stimulus onset times for trials with optogenetic manipulation and with whisker movements. Trial_OnlyWhisker_QuietTrials: data structure containing the cut signals used to compute averaged responses around whisker stimulus onset times for trials without optogenetic manipulation and without whisker movements. Trial_OnlyWhisker_WhiskingTrials: data structure containing the cut signals used to compute averaged responses around whisker stimulus onset times for trials without optogenetic manipulation and with whisker movements. Trial_OnlyOpto: data structure containing the cut signals used to compute averaged responses around optogenetic stimulus onset times in trials without whisker stimulus.