Example Datasets for Iliski - Neuronal calcium, RBC velocities and fUS responses to odorant stimuli in the mouse olfactory bulb

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Dataset information

Country of origin
Updated
2020.12.16 00:00
Created
2019.09.11
Available languages
English
Keywords
neurovascular coupling, transfer function, calcium imaging, two-photon microscopy, functional ultrasound
Quality scoring

Dataset description

Dataset containing 2 HDF5 files, one per mouse. It is intended to be used to test Iliski, a Transfer Function computation software. Iliski is available on GitLab (https://gitlab.com/AliK_A/iliski) along with the User Manual. Refer to the User Manual and to the ReadMe file for more details on Iliski. Data were already published on Zenodo (https://doi.org/10.5281/zenodo.3773863), but along an old version of the software. This upload is made for clarity purposes. Each file contains acquisitions of responses to odorant stimuli in the olfactory bulb made with : two-photon linescan microscopy (for Ca2+ and RBC velocity); functional ultrafast ultrasound, acquired from a coronal plane. HDF5 files tree is as follows : Data type Raw : straight out of our extraction software, no specific treatment applied; Aligned : every acquisition has been aligned so that the odor delivery matches the 10 s mark. Acquisitions have also been interpolated to be meaned; Delta : aligned acquisitions are subtracted with the baseline value (between 5 and 10 s); DetaOverBSL : aligned acquisitions are subtracted and then divided with the baseline value. Data source Ca : calcium data from GCamP6f expressed in the mitral cells dendritic tufts; RBC : RBC velocities in a capillary near the calcium recording site, simultaneously acquired; FUS : fUS data, coronal plane. Only in FUS folder is two different folders then : High and Lowspeed, corresponding to different filter for fUS treatment,  > 80Hz and 10-30Hz respectively. Stimulation type : Odorant_Quantity_Duration Odorant type, either Iso Amyl Acetate (AA) or Ethyl tiglate (ET); Odor quantity : measured and calibrated in volt with a photo-ionizator; Odor duration : from 5 s down to 120 ms, a single sniff for a mouse. Ca2+ : Calcium fUS : functional ultrafast ultrasound RBC : Red Blood Cell
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