Minimal dataset to test multiplexed DNA imaging (Hi-M) software pipelines

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Dataset information

Catalog

Zenodo catalog

Country of origin

EU institutions

Updated

2024.07.17 00:00

Created

2022.01.01

Available languages

English

Keywords

multiplexed imaging, FISH, chromatin

Datasource

Official portal for European data link

Quality scoring

Dataset description

This is a dataset of nuclei (DAPI), and 3 multiplexed DNA imaging cycles to test and validate processing software packages, such as pyHiM (https://github.com/marcnol/pyHiM). This dataset was acquired in a nc14 Drosophila embryo. File contents: scan_001_RT27_001_ROI_converted_decon_ch00.tif barcode 27, fiducial scan_001_RT27_001_ROI_converted_decon_ch01.tif barcode 27 scan_001_RT29_001_ROI_converted_decon_ch00.tif barcode 29, fiducial scan_001_RT29_001_ROI_converted_decon_ch01.tif barcode 29 scan_001_RT37_001_ROI_converted_decon_ch00.tif barcode 37, fiducial scan_001_RT37_001_ROI_converted_decon_ch01.tif barcode 37 scan_006_DAPI_001_ROI_converted_decon_ch00.tif DAPI scan_006_DAPI_001_ROI_converted_decon_ch01.tif DAPI, fiducial scan_006_DAPI_001_ROI_converted_decon_ch02.tif RNA To test this dataset please refer to pyHiM documentation page.

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