Human amnion epithelial cells: targeted protein profiling using Olink

These datasets contain data from quantification of proteins secreted by human amnion epithelial cells (AECs). Proteins were quantified using Olink's Target 96 Inflammation panel. Data are presented as normalized protein expression (NPX). Olink analysis 1: contains data from paired samples from 4 placenta donors on AEC-conditioned medium (AEC-CM) from 3 and 7 days of normoxic and hypoxic culture Olink analysis 2: contains data on AEC-CM from 22 placenta donors, plus a medium control samples (cell culture medium not exposed to AECs). For more info on analyzed samples, their analysis and our study setup, please see the publication below. Zeijlon L, Budhwar S, Lindau R, Bencina S, Kaipe H, Jenmalm MC, Gramignoli R and Raffetseder J (2026). Human amnion epithelial cells induce M2 macrophage polarisation partially via M-CSF secretion but independently of extracellular vesicles in vitro. Front. Immunol. 17:1723968. doi: 10.3389/fimmu.2026.1723968 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1723968/ Abstract: Pregnancy requires major immunomodulatory changes, both systemically and locally, as the maternal immune system needs to be modulated to tolerate the semi-allogeneic foetus. Decidual macrophages and stromal cells, but also foetal tissues are involved in this immune tolerance, for example by inducing M2 macrophages and regulatory T cells. However, it is so far unknown whether foetal membrane cells such as amnion epithelial cells (AECs) can influence human macrophage polarisation. In this study, a human in vitro macrophage assay was employed to demonstrate that conditioned medium (CM) from AECs derived from term placentas induces M2 macrophage polarisation, and to compare AEC culture conditions aiming for efficient M2 polarisation. Macrophage colony-stimulating factor (M-CSF), a well-known M2-inducing cytokine, was found to be secreted by AECs, and M-CSF was partly responsible for the observed M2-polarising effect of AECs. In addition, the M2-polarising effect remained after removal of extracellular vesicles (EVs) from AEC-CM, suggesting the involvement of soluble but not of EV-associated mediators. Taken together, this study shows that AECs may contribute to the induction of the vital immunotolerant environment at the foetal-maternal interface. Based on their immunomodulatory effects observed here and in in vivo studies, AECs could be harnessed as cytotherapeutics for inflammatory disorders.