TMT proteomics of erythroblasts from healthy donors and erythroblasts/ring sideroblasts from MDS-RS patients

This dataset consists of semi-quantitative 10-plex TMT proteomics analysis of FACS-separated bone marrow cells obtained from 3 healthy bone marrow donors and 3 MDS-RS patients. The objective of this data collection was to assess several parameters on how the bone marrow of MDS-RS patients differs from that of healthy donors. The samples included in this analyses were 3 samples of sorted GPA+ erythroblasts pooled from 5 healthy donors (2+2+1) and 3 samples of sorted GPA+ erythroblasts plus 3 samples of purified ring sideroblats from 3 MDS-RS patients. This dataset includes the raw mass spectra, QC and processed results. Processing: In short, FACS-separated samples were snap frozen in liquid nitrogen. Cell pellets were lysed with 4 % SDS lysis buffer and prepared for mass spectrometry using a modified version of the SP3 protein clean up and digestion protocol published by Moggridge S. et al 2018. The following files are included in the dataset: - Processed data and analysis.xlsx (2.63 MB) - Variable_list_Processed_data_and_analysis.xlsx (23 KB) - Readme for raw data .zip file.txt (562 bytes) - Readme for Processed data.xlsx file (1.07 KB) - 13553_STD_EAEHPM1_10_TMT10_HiRIEF_3-10pH_20210504_07.15.zip (7.68 GB)